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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
21/02/2014 |
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Actualizado : |
05/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FRAGA, M.; FERNÁNDEZ, S.; CAJARVILLE, C.; MARTÍNEZ, M.; ABIN-CARRIQUIRY, J.A.; ZUNINO, P. |
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Afiliación : |
MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Departamento de Microbiología Instituto de Investigaciones Biológicas Clemente Estable Montevideo Uruguay. |
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Título : |
In vitro modulation of rumen microbiota and fermentation by native microorganisms isolated from the rumen of a fed-exclusively-on-pasture bovine. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Annals of Microbiology, v. 65 n. 4, p. 2355-2362, 2015. |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 6 January 2015 /Accepted: 17 March 2015. |
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Contenido : |
Abstract:
In order to increase productive yields, modulation of rumen fermentation has been a concern in economically relevant species. The ban of antibiotics has driven attention into alternative strategies to modulate ruminal fermentation. Among these, the use of probiotics appears as an interesting approach. The objective of this work was to assess the potential of native bacteria isolated from the rumen of a fed-on-template-pasture cow to modulate fermentation in vitro and to influence the microbiota structure. Seven native ruminal bacteria strains were used in an in vitro gas production experiment. Fermentation dynamics were evaluated, and volatile fatty acids (VFA) and methane were quantified by high-performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. Microbiota structure was assessed by pyrosequencing and methanogens were quantified by quantitative PCR (qPCR). Added strains modulated fermentation dynamics and VFA synthesis. Neither the general structure of the fermenters microbiota, numbers of methanogenic microorganisms nor methane production were altered by added bacteria. However, addition of two strains reduced the volume of gas produced from soluble carbohydrates, while one of them reduced the ratio of gas production in this phase; this was supported by a VFA concentration diminution (4 h incubation) in almost every treatment. Gas produced by fermentation of non-soluble carbohydrates and its fermentation ratio were enhanced by several strains. Also, the abundances of Lachnospiraceae, Veillonellaceae, Rikenellaceae and Succinivibrionaceae were affected by strain supplementation. Modulation of fermentation by selected ruminal native bacteria was achieved, probably enhancing the fermentation of non-soluble carbohydrates. This study represents a new approach in the knowledge related to the use of probiotics in ruminants. MenosAbstract:
In order to increase productive yields, modulation of rumen fermentation has been a concern in economically relevant species. The ban of antibiotics has driven attention into alternative strategies to modulate ruminal fermentation. Among these, the use of probiotics appears as an interesting approach. The objective of this work was to assess the potential of native bacteria isolated from the rumen of a fed-on-template-pasture cow to modulate fermentation in vitro and to influence the microbiota structure. Seven native ruminal bacteria strains were used in an in vitro gas production experiment. Fermentation dynamics were evaluated, and volatile fatty acids (VFA) and methane were quantified by high-performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. Microbiota structure was assessed by pyrosequencing and methanogens were quantified by quantitative PCR (qPCR). Added strains modulated fermentation dynamics and VFA synthesis. Neither the general structure of the fermenters microbiota, numbers of methanogenic microorganisms nor methane production were altered by added bacteria. However, addition of two strains reduced the volume of gas produced from soluble carbohydrates, while one of them reduced the ratio of gas production in this phase; this was supported by a VFA concentration diminution (4 h incubation) in almost every treatment. Gas produced by fermentation of non-soluble carbohydrates and its fermentation ratio were enhanced by seve... Presentar Todo |
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Palabras claves : |
FERMENTATION MODULATION; IN VITRO GAS PRODUCTION; MICROBIOTA RUMINAL; NATIVE RUMINAL MICROORGANISMS; PROBIÓTICOS; PROBIOTICS; RUMEN MICROBIOTA. |
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Thesagro : |
PRODUCCIÓN ANIMAL. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02838naa a2200289 a 4500
001 1049916
005 2019-11-05
008 2015 bl uuuu u00u1 u #d
100 1 $aFRAGA, M.
245 $aIn vitro modulation of rumen microbiota and fermentation by native microorganisms isolated from the rumen of a fed-exclusively-on-pasture bovine.
260 $c2015
500 $aArticle history: Received: 6 January 2015 /Accepted: 17 March 2015.
520 $aAbstract: In order to increase productive yields, modulation of rumen fermentation has been a concern in economically relevant species. The ban of antibiotics has driven attention into alternative strategies to modulate ruminal fermentation. Among these, the use of probiotics appears as an interesting approach. The objective of this work was to assess the potential of native bacteria isolated from the rumen of a fed-on-template-pasture cow to modulate fermentation in vitro and to influence the microbiota structure. Seven native ruminal bacteria strains were used in an in vitro gas production experiment. Fermentation dynamics were evaluated, and volatile fatty acids (VFA) and methane were quantified by high-performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. Microbiota structure was assessed by pyrosequencing and methanogens were quantified by quantitative PCR (qPCR). Added strains modulated fermentation dynamics and VFA synthesis. Neither the general structure of the fermenters microbiota, numbers of methanogenic microorganisms nor methane production were altered by added bacteria. However, addition of two strains reduced the volume of gas produced from soluble carbohydrates, while one of them reduced the ratio of gas production in this phase; this was supported by a VFA concentration diminution (4 h incubation) in almost every treatment. Gas produced by fermentation of non-soluble carbohydrates and its fermentation ratio were enhanced by several strains. Also, the abundances of Lachnospiraceae, Veillonellaceae, Rikenellaceae and Succinivibrionaceae were affected by strain supplementation. Modulation of fermentation by selected ruminal native bacteria was achieved, probably enhancing the fermentation of non-soluble carbohydrates. This study represents a new approach in the knowledge related to the use of probiotics in ruminants.
650 $aPRODUCCIÓN ANIMAL
653 $aFERMENTATION MODULATION
653 $aIN VITRO GAS PRODUCTION
653 $aMICROBIOTA RUMINAL
653 $aNATIVE RUMINAL MICROORGANISMS
653 $aPROBIÓTICOS
653 $aPROBIOTICS
653 $aRUMEN MICROBIOTA
700 1 $aFERNÁNDEZ, S.
700 1 $aCAJARVILLE, C.
700 1 $aMARTÍNEZ, M.
700 1 $aABIN-CARRIQUIRY, J.A.
700 1 $aZUNINO, P.
773 $tAnnals of Microbiology$gv. 65 n. 4, p. 2355-2362, 2015.
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| 122. |  | MÉNDEZ, R.; DEAMBROSI, E. Evaluación de fuentes de nitrógeno de liberación lenta. Manejo de suelos y nutrición vegetal. III. Fertilización. ln: INIA TREINTA Y TRES. PROGRAMA NACIONAL DE ARROZ. Investigaciones agronómicas: reporte técnico anual 2003. Área cultivos. Treinta y Tres (Uruguay): INIA, 2003. cap. 3, p. 31-33. (INIA Reporte Técnico Anual; 01)| Biblioteca(s): INIA Treinta y Tres. |
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| 124. | | CASTERÁ, F.; ROEL, A.; DEAMBROSI, E.; MÉNDEZ, R. Respuesta del arroz a la fertilización nitrogenada en dos momentos de inundación en la zona Este del Uruguay. [Resumen]. ln: Conferencia Internacional de Arroz de Clima Templado, 3., 2003, Punta del Este, Uruguay Resúmenes. Montevideo (Uruguay): ACA; INIA; GMA; FLAR, 2003. p. 54. "Instituto Nacional de Investigación Agropecuaria, Uruguay (INIA); Asociación de Cultivadores de Arroz (ACA); Gremial de Molinos Arroceros (GMA); Fondo Latinoamericano de Arroz de Riego (FLAR)"| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Tacuarembó; INIA Treinta y Tres. |
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| 128. | | MÉNDEZ, R.; DEAMBROSI, E.; BLANCO, P.H.; SALDAIN, N.E.; PÉREZ DE VIDA, F.; GAGGERO, M. Tecnología para la siembra del cultivo de arroz con reducción o eliminación del laboreo para la zona Este del Uruguay. [Resumen]. ln: Conferencia Internacional de Arroz de Clima Templado, 3., 2003, Punta del Este, Uruguay Resúmenes. Montevideo (Uruguay): ACA; INIA; GMA; FLAR, 2003. p. 64 "Instituto Nacional de Investigación Agropecuaria, Uruguay (INIA); Asociación de Cultivadores de Arroz (ACA); Gremial de Molinos Arroceros (GMA); Fondo Latinoamericano de Arroz de Riego (FLAR)"| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Treinta y Tres. |
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| 130. | | MENDEZ, R.; ROEL, A.; FUREST, J. Agroclimatología. ln: INIA TREINTA Y TRES. Arroz: resultados experimentales 2001-2002. Treinta y Tres (Uruguay): INIA, 2002. cap. 1, p. 1-2. (INIA Serie Actividades de Difusión; 292)| Biblioteca(s): INIA Treinta y Tres. |
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| 135. | | BLANCO, P.H.; GAGGERO, T; PÉREZ DE VIDA, F.; ÁVILA, S.; LAVECCHIA, A.; MARCHESI, C.; ZORRILLA DE SAN MARTÍN, G.; ACEVEDO, A.; DEAMBROSI, E.; MÉNDEZ, R. INIA OIJMAR (L 3000): Nueva variedad de arroz de alto rendimiento. El País Agropecuario, 2002, v. 7, no. 91, p. 25-28| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Tacuarembó. |
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